Courses
Interpretation
of Macromolecular Structure Data 8 hours $2000 + travel costs
Introduction to Crystallization Trials 8 hours
$2000 + travel costs
Advanced Crystallization Techniques 24 hours $6000
+ travel costs and lab materials
Introduction to X-ray Crystallography Data Collection
Techniques 8 hours $2000 + travel costs
1.
Interpretation of Macromolecular Structure Data
This course is designed to bring molecular biology and genetics
laboratory personnel quickly up to speed in evaluating and interpreting
macromolecular structure data. The information covered in this course
will enable the investigator to evaluate structural studies publications
more efficiently, work with structure model files using Rasmol and
other graphics viewers, and to evaluate the quality of the data
based upon the commonly reported NMR and x-ray crystallographic
statistics and data collection procedures. A primary goal of this
course is to prevent misinterpretation of structural data, including
over interpretation of unresolved regions of the model.
Principles
of DNA, RNA and protein structure
X-ray crystallography analysis methods
NMR analysis methods
PDB and mmCIF model coordinate files
Interpretation of coordinate files
Rasmol graphics viewer
Trends in structural biology
8-hour
course. $2000. Travel costs not included. Maximum class size 30
students.
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2.
Introduction to Crystallization Trials
This
course is designed to train laboratory personnel to obtain crystals
of macromolecules using several commercially available screens by
the hanging drop method. The course covers the theoretical basis
of crystallization, the principles of the hanging drop method, commercial
products available to implement this method, hands-on experience
setting up crystallization trials, principles of crystallization
trial evaluation, and hands-on observation and evaluation of crystallization
trays. A primary goal of this course is to enable laboratories with
no structural biology background to efficiently embark on a crystallization
trial of their favorite macromolecule, thus exploring the feasibility
of further x-ray crystallographic studies.
Crystallization
theory
Screening theory
Principles of the hanging drop method
Commercial products available
Workshop: Setting up crystallization trials
Principles of crystallization trial evaluation
Workshop: Evaluation and scoring of crystallization trials
Trends in crystallization practices
8-hour
course: $2000. Travel costs not included. Maximum class size 20
students.
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3.
Advanced Crystallization Techniques
This course is designed to train laboratory personnel to obtain
crystals of macromolecules using the current crystallization techniques.
The course is expressly designed for laboratories that have significant
quantities (at least 5 mg) of the purified target macromolecule
available for study. The course covers the theoretical basis of
crystallization, the principles of crystallization setups, commercial
products available, design and creation of crystallization setups,
hands-on experience setting up crystallization trials by different
methods, principles of crystallization trial evaluation, and hands-on
observation and evaluation of crystallization trays. A primary goal
of this course is to enable laboratories with minimal structural
biology background to efficiently embark on an x-ray crystallographic
analysis of their favorite macromolecule.
Crystallization theory
Principles of crystallization trials
Principles of the hanging and sitting drop methods
Principles of the capillary diffusion and button methods
Commercial products available
Home-made products
Workshop: Setting up crystallization trials
Principles of crystallization trial evaluation
Workshop: Evaluation and scoring of crystallization trials
Trends in crystallization practices
24-hour course: $6000. Travel costs not included. Maximum class
size 15 students. The laboratory is expected to acquire the basic
crystallization equipment and supplies.
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4.
Introduction to X-ray Crystallography Data Collection Techniques
This
course is designed to introduce laboratory personnel to x-ray crystallographic
data collection techniques and their requirements. The course covers
rotating anode and synchrotron x-ray sources, image plate and CCD
detectors, desired crystal qualities, obtaining beam time, principles
of data collection, and an overview of the structure determination
process. A primary goal of this course is to enable laboratories
that have embarked upon crystallization experiments to intelligently
explore avenues of further x-ray crystallographic studies.
The crystallographic process
Rotating anode x-ray sources
Synchrotron x-ray sources
Data collection using image plates and CCD detectors
Desired crystal qualities
Principles of data collection
Obtaining data collection time
Structure determination overview
Trends in data collection practices
8-hour
course: $2000. Travel costs not included. Maximum class size 30
students.
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